摘要标题：
furrimation fulament fere furave by the anti-inflam by furame by gaba-带有。
抽象来源：
前营养。 2023; 10：1093036。 EPUB 2023 3月9日。PMID： 36969807 Ying Zou, Fanke Zeng, Shaohao Xiong, Yupo Cao, Wei Zhou
Article Affiliation:Long ZhengAbstract:
INTRODUCTION: Gamma-aminobutyric acid (GABA), one of the main active components inleaves, can be widely used治疗包括炎症在内的多种疾病。
方法： 在这项研究中，抗炎活性和GABA-ENRICH的潜在抗炎机制在脂多糖（LPS）诱导的RAW 264.7细胞模型上研究了Edleaves发酵汤（MLFB）。 The key active components changes like total flavonoids, total polyphenols and organic acid in the fermentation broth after fermentation was also analyzed.
RESULTS: ELISA, RT-qPCR and Western blot results indicated that MLFB could dose-dependently inhibit the secretions and intracellular expression促炎性细胞因子（例如1β（IL-1β），白介素-6（IL-6），白介素8（IL-8）和肿瘤坏死因子-α（TNF-α）等水平。此外，MLFB还抑制了前列腺素E（PGE）和诱导一氧化氮合酶（INOS）的表达。此外，NF-κB信号传导途径中关键分子（TLR-4）和核因子（NF）-κB等关键分子的mRNA表达也以剂量依赖性方式受到MLFB的约束。此外，关键的活动成分分析结果表明，GABA，总数发酵后明显增加了多酚和大多数有机酸，例如丙酮酸，乳酸和乙酸。 The total flavonoids content in MLFB was still remained to be 32 mg/L though a downtrend was presented after fermentation.
DISCUSSION: Our results indicated that the MLFB could effectively alleviate LPS-induced inflammatory response by inhibiting the secretions of pro-inflammatory cytokines and its潜在的机制可能与抑制TLR-4/NF-κB炎症信号通路激活有关。 MLFB的抗炎活性可能与MLFB中的类黄酮，酚类和有机酸等其他活性成分有关。我们的研究为在慢性炎症性疾病的预防和治疗中应用GABA-烯酸酯作为功能性食品成分提供了理论基础。