J Ginseng Res。 2022年11月； 46（6）：750-758。 EPUB 2021 12月17日。PMID： 36312734 Hantao Zhang，Yao Cheng，Feiyan Chen，Cuihua Chen，Lin Chen，Yunan Zhao
文章隶属关系：Zhu Zhu
摘要：
Methods: The candidate protein targets of ginsenosides in brain tissues were identified by drug affinity responsive target stability (DARTS) coupled with label-free liquid chromatography-mass spectrometry （LC-MS）分析。网络药理学方法用于收集MCI的治疗靶标。基于上述重叠目标，我们在字符串数据库中建立了蛋白质 - 蛋白质相互作用（PPI）网络，并进行了基因本体论（GO）富集分析。 Finally, we assessed the effects of ginseng total saponins (GTS) and different ginsenosides on mitochondrial function by measuring the activity of the mitochondrial respiratory chain complex and performing molecular docking.
Results: We screened 2526 MCI-relat通过数据库和349个人参皂苷相关蛋白靶标的ED蛋白靶标由飞镖靶标。在这81个重叠基因的基础上，富集分析表明，线粒体在GTS介导的MCI药理过程中起着重要作用。线粒体功能分析显示GTS，Protopanaxatriol（PPT）和RD以剂量依赖性方式增加了复合物I的活性。 Molecular docking also predicted the docking pockets between PPT or Rd and mitochondrial respiratory chain complex I.
Conclusion: This study indicated that ginsenosides might alleviate MCI by targeting respiratory chain complex I and regulating mitochondrial function, supporting ginsengs认知缺陷中的治疗应用。