Click here to read the complete article.
Abstract Title:Berberine and Oligomeric Proanthocyanidins Exhibit Synergistic Efficacy Through Regulation of大肠癌中的PI3K-AKT信号通路。
摘要来源：
前oncol。 2022; 12：855860。 EPUB 2022 5月4日。PMID： 35600365“> 35600365 Yuan, Masanori Tokunaga, Yusuke Kinugasa, Ajay Goel
Article Affiliation:Keisuke Okuno
Abstract:Background: Naturally occurring dietary botanicals offer time-tested safety and anti-cancer efficacy, and a combination of certain化合物已证明可以克服难以捉摸的化学治疗性抗性，这对于提高患者的死亡率具有重要意义结直肠癌（CRC）。因此，在此，我们假设Berberine（BBR）和低聚素蛋白毒素（OPC）可能会调节在CRC中发挥出色的抗癌活性在CRC中发挥出色的抗癌途径。对患者衍生的类器官进行询问，以评估BBR和OPC对CRC的协同作用。 In addition, by performing whole genome transcriptomic profiling we identified the key targeted genes and pathways regulated by the combined treatment.
Results: We first demonstrated that OPCs facilitated enhanced cellular uptake of BBR in CRC cells by measuring the fluorescent signal of BBR in cells treated individually or their 组合。 BBR和OPC之间的协同作用是根据其抗肿瘤效应对细胞生存力的抗肿瘤作用的研究iTy，移民和入侵。此外，该组合处理增强了膜联蛋白V结合测定法中的细胞凋亡。在PI3K-AKT信号通路中鉴定出的癌基因MYB的转录组分析可能与联合处理的抗肿瘤性特性有关。 Finally, we successfully validated these findings in patient-derived CRC tumor organoids.
Conclusions: Collectively, we for the first time demonstrate that a combined treatment of BBR and OPCs synergistically promote the anti-tumorigenic properties in CRC possibly through the regulation of cellular apoptosis and PI3K-AKT信号通路中的Oncogene myb。