j Ginseng res。 2022年5月; 46（3）：396-407。 EPUB 2021 7月12日。PMID： 35600769 35600769 Huang, Sijun Park, Wookbong Kwon, Eungyung Kim, Soyoung Jang, Si-Yong Kim, Seong-Kyoon Choi, Duhak Yoon, Sung-Hyun Kim, Kangdong Liu, Zigang Dong, Zae Young Ryoo, Myoung Ok Kim
Article Affiliation:Haibo Zhang
Abstract:背景： 结直肠癌（CRC）在全球范围内具有高发病率和死亡率。 20（s） - 甘森烯糖苷RH2（G-RH2）是从中提取的天然化合物，表现出抗癌许多癌症类型的ER影响。 In this study, we demonstrated the effect and underlying molecular mechanism of G-Rh2 in CRC cells in vitro and in vivo.
Methods: Cell proliferation, migration, invasion, apoptosis, cell cycle, and western blot assays were performed to evaluate the effect of G-Rh2 on CRC cells.体外下拉测定法用于验证G-RH2和AXL之间的相互作用。转染和感染实验用于探索CRC细胞中AXL的功能。 CRC xenograft models were used to further investigate the effect of Axl knockdown and G-Rh2 on tumor growth in vivo.
Results: G-Rh2 significantly inhibited proliferation, migration, and invasion, and induced apoptosis and G/Gphase cell cycle arrest in CRC cell lines. G-RH2直接与AXL结合并抑制CRC细胞中的AXL信号通路。敲除AXL抑制了增长，迁移CRC细胞在体外和异种移植肿瘤生长体内的ATION和侵袭能力，而AXL的过表达促进了CRC细胞的生长，迁移和侵袭能力。 Moreover, G-Rh2 significantly suppressed CRC xenograft tumor growth by inhibiting Axl signaling with no obvious toxicity to nude mice.
Conclusion: Our results indicate that G-Rh2 exerts anticancer activity in vitro and in vivo by suppressing the Axl signaling pathway. G-RH2是CRC预防和治疗的有前途的候选人。