[Salvianolic A a通过调节miR-940和mir-576-5p]。
摘要来源：
Zhongguo gu shang。 2023年10月25日； 36（10）：982-9。 PMID： 37881933”> 37881933 Jie Yu, Ling-Hui Li, Wu Sun, Xin Chen, Chu-Hao Cai, Wei-Ye Zhang, Tao Han, Yu-Hui Yin, Bin Tang, Li-Guo Zhu
Article Affiliation:Jia-Wen Zhan
Abstract:OBJECTIVE: To investigate whether Salvianolic acid A (SAA) can restore cartilage endplate cell degeneration of intervertebral discs and to identify the mechanism via regulation of micro-RNA.
METHODS: Cartilage endplate cells were isolated from lumbar intervertebral disc surgical samples and were treated with serum containing a系列24、48和72小时的SAA浓度（2、5和10？m），以确定适当的剂量和治疗时间。通过Alcian蓝色染色和评估ADAMTS-5，MMP3和COL2A1的表达水平，分析了对软骨终板细胞细胞外基质降解的效应SAA对软骨终板细胞的细胞外基质降解的作用。 Further, the potential target miRNAs were preliminarily screened by micro-RNA sequencing combining qRT-PCR and Western blot, and then, the miRNAs mimics and inhibitors were used to verify the regulatory effect of SAA on potential target miRNAs.
RESULTS: The 10μM SAA treatment for 48 h显着增强了软骨终板细胞的生存力，并增加了受IL-1β抑制的COL2A1表达和糖胺聚糖的积累，并降低了IL-1βONADAMTS-5和MMP3的作用。基于微RNA测序和VENNY分析的筛选分析确定了下游微RNA，包括miR-940和miR-576-5p。然后，将miR-940-MIMIC或miR-576-5p模拟转染到CEPC中。 Compared with the SAA group, the expression of ADAMTS-5 and MMP3 increased significantly and the expression of COL2A1 obviously decreased after overexpression of miR-940 or miR-576-5p in CEPCs.
CONCLUSION: Salvianolic acid A attenuated the IL-1β-induced extracellular通过靶向miR-940和miR-576-5p。