雄激素抑制静态机械压力诱导的椎间盘变性

cunxin Zhang

目的：

方法： heaosein（He）蓝色和免疫荧光染色用于鉴定NPC。使用自制细胞加压装置构建了NPC凋亡模型。使用试剂盒检测到增殖活性，活性氧（ROS）含量和凋亡率。使用蛋白质印迹检测到相关蛋白的表达。使用自制的尾骨应力装置构建大鼠尾骨IDD模型。 HE staining and safranine O-fast green FCF cartilage staining were used to observe the degeneration degree of the intervertebral disk.

RESULTS: ADR inhibits static mechanical pressure-induced apoptosis and ROS accumulation in NPCs and improves cell viability. ADR can promote the expression of Heme oxygenase-1 (HO-1), p-Nrf2, p-p38, p-Erk1/2, p-JNK, and other proteins, and its effects can be blocked by inhibitors of the above proteins.

CONCLUSION: ADR can inhibit IDD通过激活MAPK/NRF2/HO-1信号通路并抑制NPC中的静态机械压力诱导的ROS积累。