通过抑制db /db小鼠中的smad3表达来对formononetin的肾脏保护作用

jiawei lv

目的： glomerular sclorisos和肾纤维纤维病是最重要的疾病。 SMAD3在高葡萄糖诱导的肾小管纤维化中扮演拮抗作用，这是糖尿病肾病（DN）的重要治疗靶标。 Formononetin（FMN）对包括DN在内的糖尿病血管并发症。但是，它是否通过调节SMAD3来起着抗纤维化作用，尚不清楚。 The purpose of this study was to evaluate the renoprotective effect of FMN by suppressing smad3 expression in db/db mice.

Methods: FMN was orally administered to db/db mice with a dose of 25 or 50 mg/kg/day for 8 weeks.在研究结束时，收集了血清，尿液和肾脏样品进行生化和病理检查。 The expressions of proteins and mRNA associated with renal fibrosis were determined by biochemical, histological, immunofluorescence, and real-time PCR analysis.

Results: The results showed that FMN substantially improved the glucolipid metabolism, reduced the oxidative stress, and protected renal function in DB/DB小鼠。同时，SMAD3的蛋白质和mRNA表达及其相关调节因子r矩阵沉积被显着抑制。