纤维补充影响人类肠道微生物组的系统发育结构和功能能力：随机对照试验的随访。
摘要来源：
am j clin Nutr。 2015年1月； 101（1）：55-64。 EPUB 2014 11月12日。PMID： 25527750 25527750 Hooda, Jennifer M Brulc, George C Fahey, Kelly S Swanson
Article Affiliation:Hannah D Holscher
Abstract:BACKGROUND: In our published randomized, double-blind, placebo-controlled, 3-period crossover trial, healthy adult men (n = 21)消耗的条没有含有补充纤维（安慰剂； NFC），多脱粉（21 g/d）和可溶性玉米纤维（SCF; 21 g/d）的棒。在第16天到第21天之间收集粪便标本，以进行发酵终产物分析和16S核糖体RNA细菌细菌分类识别的Al基因扩增。 Fiber supplementation decreased fecal putrefaction compounds and shifted abundances of several bacterial taxa.
OBJECTIVE: The objective was to perform whole-genome shotgun 454 pyrosequencing on the same fecal specimens collected in that clinical trial to obtain comprehensive fecal bacterial genome sequencing coverage and explore the full range of bacterial genetic information in the fecal microbiome, thereby using a systematic approach to study the impact of dietary fiber supplementation on fecal metabolites, bacterial taxa, and bacterial metagenomes.
DESIGN: Fecal samples were subjected to全基因组shot弹枪454焦磷酸测序，以识别存在的粪便细菌种群及其功能性遗传能力。
结果： 全部shotgun shotgun shotgun shotgun sothgun sothgun sotchgun sotchgun soteg序列序列CING的结果表明，纤维的消耗转移了细菌群：浓度比，与NFC相比，多脱脱糖和SCF的杆菌分别增加了12±2％和13±2％的相对丰度。双变量相关性在菌叶植物之间显示出正相关：公司的比率和总饮食纤维摄入量，而不是体重指数。 Bray-Curtis距离的主要坐标分析表明，与NFC相比，在食用纤维（多脱脱糖和SCF合并）的参与者中，细菌基因组成更相似。 Shifts in bacterial gene abundances after polydextrose and SCF supplementation included genes associated with carbohydrate, amino acid, and lipid metabolism, as wellas metabolism of cofactors and vitamins.
CONCLUSION: This study conveys novel information about the impact of dietary fiber补充系统发育结构和功能能力健康成年人的粪便微生物组。