摘要来源： virol J. 2019 07 31; 16（1）：94。 EPUB 2019年7月31日PMID： 31366366“> 31366666 Tzyy-Rong Jinn Wen-Yu Lin BACKGROUND: Although enterovirus 71 (EV71) is an important public health threat, especially in the Asia-Pacific region, there are still no effective drugs or vaccines to treat and prevent EV71 infection.因此，开发针对EV71的预防性和治疗剂至关重要。罗斯马林酸（RA）是一种植物化学物质，已被发现以POSsess a broad spectrum of biological activities. METHODS: The virucidal effects of RA on EV71 were determined by MTT, western blot, median cell culture infectious dose, apoptosis detection, plaque reduction, semi-quantitative real-time polymerase chain reaction, immunofluorescence detection, molecular docking analysis, and mouse protection assay. RESULTS: RA showed a strong protective effect against EV71 infection in human rhabdomyosarcoma cells when the multiplicity of infection was 1, with a low ICvalue (4.33 ± 0.18 μM) and high therapeutic index （340）。 RA不仅保护细胞免受EV71诱导的细胞病变作用，还来自EV71诱导的细胞凋亡。成熟时间分析的结果表明，在病毒感染的早期，RA的抑制活性最高。与此相一致，EV71在病毒INF的早期阶段的感染性在用RA处理的新生小鼠中也观察到摄取受到限制。此外，分子对接预测，RA可以替换VP1胶囊结合的疏水口袋内的自然袖珍因子。
结论： span> 这项研究表明，RA的潜力可以防止抗抗病变剂，以防止抗抗病变的抗病经验。可以有效地减少病毒感染早期的EV71感染。