银杏提取物通过激活AMPK-NRF2-NQO-1轴。 2022年12月30日。EPUB2022 12月30日。PMID： 36583518 36583518 Zhu, Zhengqi Cheng, Ling Zhu, Michael Murray, Fanfan Zhou
Article Affiliation:Yue Li
Abstract:OBJECTIVES: Retinal Müller glial cell loss is almost involved in all retinal diseases, especially diabetic retinopathy (DR).氧化应激显着有助于Müller神经胶质细胞损失的发展。据报道，银杏提取物（GBE）具有抗氧化特性，有益于治疗人类视网膜疾病。然而，对其在müller神经胶质细胞中的作用知之甚少。这项研究调查了保护性效果t of GBE (prepared from ginkgo biloba dropping pills) in human Müller glial cells against tert-butyl hydroperoxide (t-BHP)-induced oxidative stress and its underlying molecular mechanism.
METHODS: MIO-M1 cells were pretreated with or without GBE prior to the暴露于T-BHP诱导的氧化应激。随后评估了细胞活力，细胞死亡特征和脂质过氧化。 Protein expression of the key anti-oxidative signalling factors were investigated.
KEY FINDINGS: We showed that GBE can effectively protect human MIO-M1 cells from t-BHP-induced oxidative injury by improving cell viability, reducing intracellular ROS accumulation and suppressing lipid peroxidation, which effect is likely通过激活AMPK-NRF2-NQO-1抗氧化受访者轴介导的轴。
结论： 我们的研究是首次的研究揭示了GBE在保护人类视网膜神经胶质细胞损失免受氧化应激的巨大潜力。 GBE可能用于预防人类视网膜疾病，特别是Dr。