通过姜黄素通过糖尿病大鼠晶状体中α-晶状蛋白伴侣活性的调节。
摘要来源：
mol vis。 2005; 11：561-8。 EPUB 2005年7月26日。PMID： 16088325 16088325 Yadagiri Reddy, G Bhanuprakash Reddy
Article Affiliation:National Institute of Nutrition, Hyderabad, India.
Abstract:PURPOSE: A decline in the chaperone-like activity of eye lens alpha-crystallin in diabetic conditions has been reported. In this study, we investigated whether curcumin, a dietary antioxidant, can manipulate the chaperone-like activity of alpha-crystallin in diabetic rat lens.
METHODS: A group of rats received ip injection of streptozotocin (STZ; 35 mg/kg body weight in缓冲液）诱发高血糖，而另一个一组大鼠仅接收缓冲液作为车辆并用作对照。 STZ处理的大鼠分配给3组，并分别喂养无姜黄素或0.002％或0.01％姜黄素。通过缝隙灯生物显微镜监测高血糖引起的白内障进展。在8周结束时，牺牲了动物并收集镜片。通过凝胶过滤分离出一组合并透镜的α-和α-晶状体。 Chaperone activity, hydrophobicity, and secondary and tertiary structure of alphaH- and alphaL-crystallins were assessed by light scattering/spectroscopic methods.
RESULTS: A decrease in chaperone-like activity of alphaH- and alphaL-crystallins was observed in经STZ治疗的糖尿病大鼠。由于高血糖引起的伴侣样活性下降与疏水性降低以及α-和α-晶状体的二级和三级结构改变有关。有趣的是，Alphah-与未经处理的糖尿病大鼠透镜中的α-和α-晶状体相比，从姜黄素喂养的糖尿病大鼠透镜中分离出的α-晶状体表现出改善的伴侣样活性。姜黄素的喂养阻止了由于STZ诱导的高血糖引起的疏水性和结构变化的改变。发现姜黄素对姜黄素的功能和结构特性的调节比α-晶状蛋白大于α-晶状体。在糖尿病大鼠透镜中，α-晶状体蛋白，尤其是α-晶状体的伴侣活性的丧失至少部分归因于氧化应激的增加。 Being an antioxidant, curcumin feeding has prevented the loss of alpha-crystallin chaperone activity and delayed the progression and maturation of diabetic cataract.
CONCLUSIONS: We demonstrate that curcumin, at the levels close to dietary consumption, prevented the loss of chaperone-like activity alpha-crystallin v由于大鼠镜头中的糖尿病而引起的is-a-a-vis发生。