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Abstract Title:Thionin-like peptide from Capsicum annuum fruits: mechanism of action and synergism氟康唑对念珠菌物种。
抽象来源：
bmc microbiol。 2016; 16（1）：12。 EPUB 2016 1月27日。PMID： 26819228 26819228 Rosana Rodrigues, Fernanda G Trindade, Maura Da Cunha, Valdirene M Gomes
Article Affiliation:Gabriel B Taveira
Abstract:BACKGROUND: Thionins are a family of plant antimicrobial peptides (AMPs), which participate in plant defense system反对病原体。在这里，我们描述了先前从辣椒酸果实中分离出的类似Cathi Thionin的作用机制的某些方面。类似硫宁的PE对氟康唑评估，将抗皮质提交给针对IC50测定和协同作用的念珠菌物种的抗菌活性测定。 Viability and plasma membrane permeabilization assays, induction of intracellular ROS production analysis and CaThi localization in yeast cells were also investigated.
RESULTS: CaThi had strong antimicrobial activity against six tested pathogenic Candida species, with IC50 ranging from 10 to 40μg.ml（-1）。 Cathi对念珠菌物种的抗菌活性是念珠菌的。此外，Cathi在所有测试的酵母中引起质膜通透性，并仅在热带念珠菌中诱导氧化应激。 Cathi细胞内定位在白色念珠菌和热带梭菌中，但是将其定位在热带梭菌中的核中，这表明可能是核靶标。 Cathi与氟康唑协同进行，抑制所有测试的酵母菌，在副梭状芽胞杆菌中达到100％抑制作用。抑制协同对的浓度范围从Cathi IC50低1.3到4.0倍，从零氟康唑IC50低于零到2.0倍。酵母。