j Lasers Med Sci。 2019; 10（3）：171-178。 EPUB 2019年7月6日。PMID： 317499941 31749941 Ezedin Mohajerani，Mohammad Hosein Ghazimoradi，Reza Shiari
文章隶属关系：Shirin farivar
摘要：
受损软骨细胞对自我更新的能力有限，这是由于其特定的组织结构，类似于其他组织，可能导致诸如诸如其他组织结构，例如可能导致诸如诸如诸如其特定的组织结构之类的问题。在这项工作中，使用激光光生物调节和有趣的生长因子来源称为Synovial fluid, we analyzed the chondrogenic marker genes in treated hair follicle dermal papilla cells as an accessible source of cells with relatively high differentiation potential.Dermal papilla cells were isolated from rat whisker hair follicle (Rattus norvegicus) and established cell cultures were treated with a laser (gallium aluminum arsenide diode Laser (λ=780 NM，30 MW）在5 j/cm），滑液和两者的组合。 1、4、7和14天后，评估了形态学的变化，并通过定量实时聚合酶链反应评估了四个软骨细胞标记基因（和）的表达水平。该反应监测了用激光辐照治疗细胞可以加速细胞的增殖率。用滑液处理的细胞的形态发生了很大的改变，就像在第四天一样，它们看起来像是培养的关节软骨细胞。基因表达分析表明，所有基因都被上调直到治疗后的第14天，尽管在所有细胞组中并非平等。 Moreover, the cell groups treated with both irradiation and the synovial fluid had a significantly augmented expression in gene markers.Based on the gene expression levels and the morphological changes, we concluded that the synovial fluid can have the potential to make the dermal papilla cells to most likely mimic the chondrogenic and/or osteogenic differentiation, although this process seems to be augmented by the irradiation of the low-level激光。