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Abstract Title:Chebulinic acid isolated from aqueous extracts ofRetz inhibitsinfection by potential binding to Cag A protein
摘要来源：
前微生物。 2024; 15：1416794。 EPUB 2024 10月2日。PMID： 39421559 39421559 Zhixiang Zhu，Qingwei Li，Qingchang Chen，Ruixia Wei，Zhong Feng，Guimin Zhang，Meicun Yao
文章隶属关系：ling OU
摘要：
中国因其抗氧化剂，免疫调节，抗菌和抗炎特性而闻名。 Chebulinic Acid，源自Retz水提取物，以其抗炎特性。 However, its potential as an anti-(HP) agent has not been fully explored.
METHODS: Herein, we extracted the main compound fromRetz using a semi-preparative liquid chromatography (LC) system and identified compound 5 as chebulinic acid through Ultra-high performance liquid chromatography-MS/MS （UPLC-MS/MS）和核磁共振（NMR）。 To evaluate its role, we conducted minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) assays, scanning electron microscope (SEM) imaging, inhibiting kinetics curves, urea fast test, cell counting kit-8 (CCK-8) assay, western blot analysis, griess reagent system, and molecular docking.
结果： 我们的结果表明，切布林酸有效抑制了HP菌株ATCC 700392的生长，损坏了HP结构并表现出选择性抗菌活性Wi影响正常上皮细胞GES-1。重要的是，它抑制了细胞毒素相关基因A（CAG A）蛋白的表达，这是HP感染的关键因素。分子对接分析分析预测蛋白质和CAG之间的较强亲和力（-9.7 kcal/mol）。
结论： 总体上，我们的发现总的来说，我们的发现表明chebulinic ate the to and tright to and trige HP感染。它还抑制CAG蛋白质。这些结果突出了Chebulinic Acid对HP感染的潜力。