摘要来源： world j胃肠道。 2017年9月14日； 23（34）：6252-6260。 PMID： 28974891 Hong-Xia Chen, Pan Ren, Yu-Lin He, Pei Hu, De-Qiang Ma, Jie Luo, Zhong-Ji MengArticle Affiliation: Zhi-Qiang Wei AIM: To investigate the potential effect of curcumin on hepatitis B virus （HBV）共价闭合的圆形DNA（CCCDNA）和基础机制。 方法： a hepg2.2.2.15 hepg2.2.15细胞系稳定转染了用HBV转染了HBVELISA评估了姜黄素和HBV表面抗原（HBSAG）和E抗原（HBEAG）表达水平。细胞内HBV DNA复制中间体和CCCDNA分别通过Southern印迹和实时PCR检测。组蛋白H3和H4的乙酰化水平通过蛋白质印迹测量。通过染色质免疫沉淀（芯片）测定检测H3/H4结合的CCCDNA。脱乙酰基酶抑制剂trichostatin A和丁酸钠用于研究姜黄素作用机理。 Additionally, short interfering RNAs (siRNAs) targeting HBV were tested along with curcumin. RESULTS: Curcumin treatment led to time- and dose-dependent reductions in HBsAg and HBeAg expression and significant reductions in intracellular HBV DNA replication intermediates and HBV CCCDNA。用20μmol/L姜黄素处理2 d后，HEPG2.2.2.15细胞中的HbSAG和CCCDNA水平降低了57.7％（p <0.01）和75.5％（p <0。与未处理细胞的水平相比，分别为01）。同时，在用姜黄素治疗后，还检测到组蛋白H3乙酰化水平的时间和剂量依赖性降低，并伴有H3和H4结合的CCCDNA的降低。此外，脱乙酰基酶抑制剂trichostatin A和丁酸钠可以阻止姜黄素的作用。 Additionally, transfection of siRNAs targeting HBV enhanced the inhibitory effects of curcumin. CONCLUSION: Curcumin inhibits HBV gene replication via down-regulation of cccDNA-bound histone acetylation and has the potential to be developed as a cccDNA-targeting丙型肝炎的抗病毒剂