摘要来源： br j Pharmacol。 2014年1月； 171（1）：237-52。 PMID： 24117426“ Shainn-Wei Wang, Wei-Chun Chen, Chun-Kuang Lin, Yu-Hsuan Wu Jin-Ching Lee BACKGROUND AND PURPOSE: This study aimed to evaluate the anti-hepatitis C virus (HCV) activity of Andrographolide，一种从雄激素paniculata中提取的二萜类内酯，以识别其抗病毒作用所涉及的信号传导途径。 实验方法： 使用HCV复制子和HCVCC感染性系统，我们通过测量蛋白质和RNA水平来鉴定雄激素的抗HCV活性。记者活性测定法用于确定抗HCV药物的转录调节。 A specific inhibitor and short hairpin RNAs were used to investigate the mechanism responsible for the effect of andrographolide on HCV replication. KEY RESULTS: In HCV replicon and HCVcc infectious systems, andrographolide time- and dose-dependently suppressed HCV replication.当与IFN-α（靶向HCV NS3/4A蛋白酶（Telaprevir）或NS5B聚合酶（PSI-7977））的IFN-α结合使用时，Andrographolide表现出显着的协同作用。 Andrographolide上调了Haeme氧酶-1（HO-1）的表达，导致其代谢产物双列酰丁的量增加，发现通过抑制HCV复制促进抗病毒反应并抑制NS3/4A蛋白酶活性。值得注意的是，这些抗病毒作用被HO-1特异性抑制剂或HO-1基因敲低减弱，表明HO-1有助于雄摄醇的抗HCV活性。 Andrographolide activated p38 MAPK phosphorylation, which stimulated nuclear factor erythroid 2-related factor 2 (Nrf2)-mediated HO-1 expression, and this was found to be associated with its anti-HCV activity. CONCLUSIONS AND IMPLICATIONS: Our results demonstrate that andrographolide has the potential to控制HCV复制，并建议针对NRF2-HO-1信号通路可能是药物开发的有前途的策略。