红色发光二极管治疗最大程度地减少了抗逆转录病毒利托那韦对成骨细胞的功能有害影响。
摘要来源：
lasers med Sci。 2024年1月17日； 39（1）：34。 EPUB 2024 1月17日。PMID： 38231300 Pessoa，Cintia Kazuko Tokuhara，Mariana Liessa Rovis Sanches，Cassia Senger，Maria aparecida andrade Andrade Moreira，Rodrigo Cardoso Oliveira
文章隶属关系：MatheuseusmenãoMenãoMenãoMenãoMochetti
MOCHETTI
rast_ P> 长期人类免疫缺陷病毒（HIV）感染的患者被认为患有骨质疏松症的风险更高。在导致这些患者降低骨骼健康的各种原因中，使用抗逆转录病毒药物（ARV），尤其是蛋白酶抑制剂（PI），例如Ritonavir（RTV）。在这种情况下，出现了有效的LED治疗的IAL益处，其对骨细胞的影响目前正在广泛研究中，显示了细胞分化的调节。 However, it remains unclear if photobiostimulation might interfere with RTV effects on osteoblast differentiation.
METHODS: In the present study, we investigated the effects of red LED (625 nm) irradiation (15 mW/cm, 0.2 J/cm, and 8 mW/cm, 0.12 J/cm) on osteoblast cell line MC3T3-E1 treated with RTV (2.5, 5, and 10 μg/mL).
RESULTS: Our results indicated that red LED irradiation was able to reverse, or at least minimize, the deleterious effects of RTV on the成骨细胞。通过MTT分析评估，ARV处理5和10μg/mL（104.4％和95.01％），也不是LED方案（100.3％和105.7％）统计上改变的细胞活力，由MTT分析评估。此外，碱性磷酸酶活性和矿化的成骨细胞ACT降低ivity followed by ARV exposure (39.3-73%), which was attenuated by LED in more than 70% with statistical significance (p < 0.05).
CONCLUSION: In conclusion, photobiostimulation with red LED at 625 nm was associated with improved beneficial biological effects as a potential inducer of对受RTV影响细胞的成骨活性。这是第一项研究红色LED辐照比ARV处理的体外成骨细胞的研究。