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Abstract Title:Epigallocatechin-3-gallate and resveratrol attenuate hydrogen peroxide induced damage in neuronal cells.
Abstract Source:
Bratisl Lek Listy。 2023; 124(3):205-211。 PMID: 36598312“> 36598312 Yilmaz
Article Affiliation:Meltem Yilmaz Cosar
Abstract:PURPOSE: The purpose of this study is to investigate how the antioxidants resveratrol and epigallocatechin-3-gallate (EGCG) protect SH-SY5Y cells against damage caused by hydrogen peroxide (H2O2)。
方法: sh-sy5y细胞在浓度的0.1μm,1μm和10μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,1μm,尤利和各种组合。经过24小时预处理后,将细胞暴露于250μmH2O2。用MTS评估了EGCG和白藜芦醇对对过氧化氢毒性的细胞存活的影响。用caspase 3 ELISA检验测量caspase 3水平以评估生存率。 The clonogenic test was utilized to assess the colony forming capacity.
RESULTS: The MTS test revealed that pretreatment of SH-SY5Y cells for 24 hours with EGCG and Resveratrol enhanced cellular survival against hydrogen peroxide damage at all dosages (p<0.005). caspase 3 ELISA测试表明,EGCG和白藜芦醇在统计上大大降低了caspase 3水平,并通过caspase 3途径改善了细胞存活率(P <0.005)。克隆生成测试结果表明,白藜芦醇和EGCG在统计上增强了SH-SY5Y细胞可能形成菌落的潜力(p <0.005)。
c闭合: 通过降低暴露于过氧化氢损伤的caspase 3水平,EGCG和白藜芦醇促进了细胞活力(TAB。1,图3,参考文献37)。 pdf www.elis.sk中的文字关键字:epigallocatechin-3-gallate,白藜芦醇,过氧化氢,神经变性。