[来自Chia(西班牙裔Salvia)对α-淀粉酶和α-葡萄糖苷酶酶的肽分数衍生物的抑制作用。 2018年8月2日; 35(4):928-935。 EPUB 2018 8月2日。pmid: 30070884“> 30070884 Chel Guerrero, Rolffy Ortiz Andrade, David Betancur Ancona
Article Affiliation:Irving Sosa Crespo
Abstract:INTRODUCTION: biopeptides are amino acid sequences with biological functions about metabolism and carbohydrates absorption.
OBJECTIVE: the aim of this study was the evaluation of the inhibitory effect of peptide fractions derivatives of the hydrolysis of Salvia hispanica againstα-amylase and α-glucosidase enzymes to know their activity on the carbOhydrates代谢。
材料和方法: 富含蛋白质的分数通过两个酶促系统水解:Alcalase®-Flavourzyme®和Pepsin-Pancreatine。确定样品的水解等级。离心水解样品,并使用不同的切割膜对可溶部分进行超滤。确定每个部分的蛋白质含量。 An in vitro analysis was made, measuring the percentage of inhibition of the Salvia hispanica fractions against α-amylase and α-glucosidase.
RESULTS: the enzymatic system showing the highest grade of hydrolysis (63.53%) was pepsin-pancreatine.从超滤液中获得了5个肽分数:10 kDa,5-10 kDa,3-5 kDa,1-3 kDa和1 kDa。最高的蛋白质含量是这些馏分:10 kDa和5-10 kDa(分别为0.90和0.93 mg/ml),用于百事可乐 - 磷酸氨酸。 in对于10 kDa和5-10 kDa的分数分别为α-淀粉酶,获得的携带百分比分别为85.61%和79.19%。关于α-葡萄糖苷酶,最高的抑制作用是10 kDa的馏分,为96.91%。
结论:> span in> > span in chiia可以增加肽级别的糖尿病,以供糖尿病来获得自然的功能。饮食。