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Abstract Title:Tetramethylpyrazine reverses high-glucose induced hypoxic effects by negatively regulating HIF-1α诱导BNIP3表达以改善H9C2心脏肌细胞细胞凋亡。
摘要来源：
Nutr Metab（Lond）。 2020; 17：12。 EPUB 2020 JAN31。PMID： 32021640“> 32021640 Fuu-Jen Tsai，Yuan-Man Hsu，Chang-Hai Tsai，Jing-gung Chung，Jai-Sing Yang，Chih-Hsin Tang，Shulin Wang，Qiaowen Li，Chih-Yang Huang
文章伴侣：Shih-Ping Liu liu liu
糖尿病患者高度容易受到低氧损伤的影响，这与缺氧引起的BNIP3表达有关，后来激活了细胞凋亡。 ou先前的研究表明，食品调味剂四甲基吡嗪（TMP）抑制了缺氧诱导的BNIP3表达减弱心肌凋亡。 In this study, we evaluate the effect of TMP to provide protection against hypoxia aggravated high-glucose associated cellular apoptosis.Methods: The cytoprotective effect of TMP against high glucose induced cellular damages was determined on embryo derived H9c2 cardiomyoblast cells that were持续5％缺氧24小时，并受到33毫米高葡萄糖挑战的不同持续时间。 Further, the involvement of HIF-1α and BNIP3 in cellular damage and the mechanism of protection of TMP were determined by overexpression and silencing HIF-1α and BNIP3 protein expression.
Results: The results show that hypoxic effects on cell viability aggravates with high glucose challenge and this augmentative effect是m通过BNIP3在H9C2心肌细胞细胞中进行了评估。但是，TMP给药有效地逆转了增加的HIF-1α水平和BNIP3升高。 TMP改善了H9C2细胞的存活，并有效抑制了H9C2细胞的凋亡。 Further comparison on the effects of TMP on H9c2 cells challenged with high glucose and those challenged with hypoxia show that TMP precisely regulated the hypoxic intensified apoptotic effects in high-glucose condition.
Conclusion: The results clearly show that flavoring agent-TMP attenuates在高葡萄糖条件下通过不稳定HIF-1α稳定的细胞毒性通过缺氧挑战扩增。