抑制流感的一种天然小丁香甘南诺靶向病毒血凝素的病毒感染

Lianghao Huang

BACKGROUND: Given the magnitude of influenza pandemics as a threat to the global population, it is crucial to have as many prevention and treatment options as possible. piceatannol（PIC）是一种四氢羟基苯甲酸酯（Trans-3,4,3,5-四羟基苯乙烯），也被称为3-羟基白藜芦醇，它证明了许多不同的生物学活性，例如抗炎和抗病毒活性。 inthis study, the anti-influenza A virus (IAV) activities and mechanisms of PIC in vitro and in vivo were investigated in order to provide reference for the development of novel plant-derived anti-IAV drugs.

METHODS: The viral plaque assay, RT-PCR and western blot assay were used to评估PIC在体外的抗IAV效应。 PIC的抗IAV机制是通过HA合胞体测定，飞镖测定和表面等离子体共振测定法确定的。 The mouse pneumonia model combined with HE staining were used to study the anti-IAV effects of PIC in vivo.

RESULTS: PIC shows inhibition on the multiplication of both H1N1 and H3N2 viruses, and blocks the infection of H5N1 pseudovirus with low toxicity. PIC可以直接作用于IAV的包膜，以诱导IAV颗粒的破裂和失活。 PIC还可以通过与HA2而不是HA1和Cleava结合来阻止膜融合HA0的GE站点。 PIC可能与HA2的两个残基（HA2-T68和HA2-I75）相互作用，以阻止HA的构象变化以抑制膜融合。重要的是，PIC的口服治疗还明显提高了IAV感染的小鼠的存活率和病毒滴度的减少。

结论： PIC在体外和IN VIVO和IN VIVO和IN IN-IN-ICAV IAV ICAV INSCRANE中均可通过频率进行范围，从而构成了大量的抗IAV效应。因此，PIC有可能发展为一种新的广谱抗激素药物，以预防和治疗流感。