Berbamine通过抑制NF-κB发挥抗炎作用

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berbamine exerts抗激素抗炎效果通过抑制NF-beftice

细胞生理生物化学。 2017年4月26日； 41（6）：2307-2318。 EPUB 2017年4月26日。PMID： 28456802 28456802 Han-Qing Liu，Da-Jun Zhang，Yun Chen

xiao-jian jia

background/aims/aims： 这项研究旨在调查抗抑制性的berbise berbis berber a berber a berber a berber a，berber a berber a ber n ber a ber a ber n ber a）从berberis amurensis（xiao bo an）中提取的生物碱和基本机制。

方法： 巨噬细胞和中性粒细胞在体外用LP和FMLP刺激。用定量RT-PCR和ELISA评估BER对巨噬细胞中促炎性介体表达的影响。通过流式细胞术和WST-1还原测试确定BER对中性粒细胞激活和超氧化物释放的影响。通过Western印迹分析评估BER对与巨噬细胞中与炎症反应相关的信号通路激活的抑制作用。 In addition, a mouse peritonitis model was made by peritoneal injection of thioglycollat​​e medium and anti-inflammatory effects of BER were investigated in vivo by quantitative analysis of pro-inflammatory factor production and leukocyte exudation.

RESULTS: BER significantly inhibited inflammatory factor expression by LPS刺激的巨噬细胞，抑制激活和FMLP刺激的中性粒细胞的超氧化物释放。在小鼠腹膜炎模型中，BER意义重大静利抑制巨噬细胞的激活和中性粒细胞的渗出。 According to analysis, BER significantly suppressed phosphorylation of NF-κB and MAPK (JNK and ERK1/2) signaling pathways in LPS-stimulated macrophages.

CONCLUSIONS: Collectively, data from this study suggest that BER has anti-inflammatory potential, which is effected via inhibition of NF-κB和MAPK信号通路，因此有望治疗炎症性疾病。