鉴定Oldhamii提取物中成分的抑制作用，表现出对流感病毒吸附的抑制作用

Haruhito Sekizawa

We previously reported that extracts from plants of the Ericaceae genus, commonly known as the kind of blueberry, inhibited the early steps of influenza virus (IFV) infection to host cells, and that the activity was与总多酚含量相关。观察到特别有效的抑制活性。在这项研究中，我们确定了主动成分涉及抑制IFV感染。我们使用合成的吸附剂树脂列依次将提取物分解。通过HPLC分析，观察到用30％，40％和50％乙醇洗脱的级分和三个峰（峰A，B和C）的高抑制活性，通过HPLC分析在分数中鉴定出代表多酚。 Among these peaks, high inhibitory activity was detected for peak A and B, but not for peak C. These peaks were analyzed by LC/MS, which revealed that peak A contained procyanidin B2 and ferulic acid derivatives, whereas peak B contained two ferulic acid-hexosides, and peak C contained quercetin-3--rhamnoside and quercetin--pentoside--rhamnoside.众所周知，这些多酚具有抗IFV活性，但我们推测阿魏酸衍生物是抑制IFV复制早期步骤的主要原因，例如吸附或进入，观察到。