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Abstract Title:Resveratrol improves palmitic acid‑induced insulin resistance via the DDIT4/mTOR C2C12细胞中的途径。
摘要来源：
mol MedRep。2023Oct； 28（4）。 EPUB 2023 8月18日。PMID： 37594055“> 37594055 Ming Zhao, Zhimei Zhang, Xuemei Zhang, Chao Wang, Guangyao Song
Article Affiliation:Xinyan Pan
Abstract:The present study aimed to establish a model of palmitic acid (PA)‑induced insulin resistance (IR) in C2C12 cells and to determine the mechanism underlying how resveratrol (RSV) improves IR.将C2C12细胞分为对照（CON），PA，PA + RSV，PA + RSV + DNA损伤诱导的转录本4（DDIT4） - small干扰（SI）RNA和PA + RSV + RSV + MHY1485（MTor Agonist）组。确定细胞中培养基中的葡萄糖含量和甘油三酸酯含量。进行油红O染色以观察细胞的病理变化。进行了逆转录定量PCR和蛋白质印迹，以分别评估DDIT4，MTOR，p70核糖体蛋白S6激酶（p70S6K），胰岛素受体底物（IRS）−1-1，PI3K，PI3K，AKT和Glucose Transerter 4（Glutterter 4（Glut4）的mRNA和蛋白质表达水平。与CON组相比，葡萄糖摄取减少，细胞脂质沉积增加，磷酸化（P）−IRS −1，P -MTOR和P -P70S6K蛋白表达水平升高，P -PI3K，P -PI3K，P -AKT，GLUT4和DDIT4蛋白表达水平在PA组中降低。相比之下，与PA组相比，培养基葡萄糖含量和细胞脂质沉积减少，p -PI3K，p -akt，glut4和ddit4蛋白表达水平升高，p -irs -1蛋白表达水平降低，aND MTOR和P70S6K mRNA和蛋白表达水平在PA + RSV组中降低。与PA + RSV组相比，DDIT4蛋白和mRNA表达水平在PA + RSV + DDIT4 − SIRNA组中降低，但在PA + RSV + MHY1485组中没有变化。 Following transfection with DDIT4‑siRNA or treatment with MHY1485, the effects of RSV on improving IR and lipid metabolism were weakened, mTOR and p70S6K protein expression levels were upregulated, p‑PI3K, p‑AKT and GLUT4 protein expression levels were down‑regulated, p‑IRS‑1 protein expression levels were upregulated, and culture medium glucose content and cellular lipid沉积增加。总之，RSV可以通过DDIT4/MTOR/IRS -1/PI3K/AKT/GLUT4信号通路以及通过葡萄糖和脂质代谢的改善来改善C2C12细胞中PA诱导的IR。