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Abstract Title:Electroacupuncture Improves IBS Visceral Hypersensitivity by Inhibiting the Activation of内侧丘脑和前扣带回皮层中的星形胶质细胞。
抽象来源:
基于EVID的补体替代药物。 2020; 2020:2562979。 EPUB 2020 6月12日。PMID: 32617101“> 32617101 Fang Zhang, Guona Li, Zhe Ma, Huangan Wu, Yuhu Xin, Huirong Liu, Jimeng Zhao
Article Affiliation:Min Zhao
Abstract:Objective: To explore whether the effect of electroacupuncture (EA) on visceral肠易激综合征(IBS)大鼠的高敏性(VH)与内侧丘脑中星形胶质细胞激活的变化有关(MT)和前扣带回皮质(ACC)。
方法: 男性sprague-dawley大鼠被随机分为正常对照(NC)组,模型对照组,模型对照组,模型(MC)组,电动(ElectroAccucture(EartroAccucture(EaletroAccucture(EACE))组和FluoroCitrate(fuorocitrate(fca)。通过新生儿结直肠刺激建立了内脏超敏反应的模型。在EA组中,针头插入了天简(ST25)和Shangjuxu(ST37)螺旋杆的皮肤中,每天一次持续7天。 FCA组在第1、4和第7天接受了胸前注射FCA。通过腹部戒断反射(AWR)评估了内脏的超敏反应,并通过实时PCR,免疫组织化学和蛋白质的胶质酸性蛋白(GFAP)mRNA和MT和ACC中的蛋白质水平评估了蛋白质水平。组显着高于NC组,EA和FCA降低了AWR分数VH大鼠。与NC组相比,MC组大鼠MT和ACC的GFAP mRNA和ACC的蛋白水平显着增加。 After either electroacupuncture or fluorocitrate, GFAP mRNA and protein levels in the MT and ACC were both clearly reduced.
Conclusion: Electroacupuncture alleviates IBS visceral hypersensitivity by inhibiting the activation of astrocytes in the MT and ACC。