Sun Mengzhu
Abstract:OBJECTIVE: To investigate whether electroacupuncture (EA) at bilateral Tianshu (ST25) and Zusanli (ST36) acupoints could alleviate stress-induced irritable bowel syndrome (IBS) and evaluate its effect on gut microbiota and corticotropin-releasing factor (CRF).
METHODS: Thirty C57BL/6 mice were randomly divided into the normal, w避免应力（WAS），并且为+EA组（每组10只小鼠）。通过将动物暴露于IS来建立IBS的实验模型。将小鼠在双侧天苏（ST25）和Zusanli（ST36）穴位上用EA处理。进行了腹部戒断反射测试，以评估IBS中的内脏灵敏度。使用16S rRNA测序和分析分析肠道菌群。 The expression of CRF was determined using immune-ofluorescence and quantitative real-time polymerase chain reaction.
RESULTS: EA alleviated visceral hypersensitivity in a mouse model of WAS-induced IBS.它调节了由WAS诱导的肠道微生物群的营养不良。此外，它抑制了结肠组织中CRF的过度表达。
结论： 这项研究的发现表明，EA表明EA可能诱导的IBS机制可能涉及IBS机制。肠道菌群的SIS和CRF表达的抑制。