berberine通过促进维生素D受体活性来增强肠粘膜屏障功能。
摘要来源：
chin J Integn Med。 2023年4月12日。EPUB2023 APR 12.PMID： 37046128 Jia-Lin Liu, Geng-Xin Chen, Dan-Ting Shen, Wang Zhu, Xin-Lin Chen, Feng-Bin Liu, Qiu-Ke Hou
Article Affiliation:Yong-Quan Huang
Abstract:OBJECTIVE: To evaluate if berberine can act on vitamin D receptors (VDR) and thereby regulate the expression of tight junction proteins (TJPs) in irritable bowel syndrame-diarrhea-predominant (IBS-D) rats.
METHODS: The newborn rats were induced into IBS-D rat model via neonatal maternal separation combined with acetic acid化学刺激。建模后，评估了模型并大鼠S分为对照组和小ber碱治疗组（每天一次0.85、1.7和3.4 mg/kg，持续2周）。获得远端结肠并在IBS-D模型评估后分离并培养结肠上皮细胞（CEC）。在IBS-D大鼠的CEC中确定了维生素D受体反应元件（VDRE）报告基因，以分析berberine对VDRE启动子的影响。 VDR overexpression or silencing technology was used to analyze whether VDR plays a role in promoting intestinal barrier repair, and to determine which region of VDR plays a role in berberine-regulated intestinal TJPs.
RESULTS: The IBS-D rat model was successfully constructed and the symptoms were improved by berberine in a剂量依赖性方式（p <0.05）。 Berberine还有效地促进了VDRE启动子的活性（P <0.05）。 Berberine增加了IBS-D CEC中TJP的表达（P <0.05）。 VDR表达显着与正常对照组和碱性质粒组相比，转染不同域的VDR不同域后增加（所有P <0.05）。 RT-QPCR和Western印迹结果表明，与空白组相比，含有VDR组的occludin和Zonula occludens-1的表达明显更高（所有P <0.05）。 Berberine plus pCMV-Myc-VDR-N group exerted the highest expression levels of occludin and zonula occludens-1 (P<0.05).
CONCLUSION: Berberine enhances intestinal mucosal barrier function of IBS-D rats by promoting VDR activity, and the main site of action is the Vdr。
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