鲁丁蛋白和硒作为保护镉诱导的肾损害的有效抗氧化剂

Selenium and rutin alone or in combination do not have stronger protective effects than their separate effects against cadmium-induced renal damage.

Pharm Biol. 2015年10月10日：1-9。 Epub 2015 Oct 10. PMID: 26455473

Sunny O Abarikwu, Olusegun L Adebayo, Chiagoziem A Otuechere, Blessing O Iserhienrhien, Temitope A Badejo

Sunny O Abarikwu

CONTEXT: Selenium (Se) and rutin (RUT) are antioxidants that protect against tissue damage.

OBJECTIVE: In this study, the separate and combine protective effects of RUT and Se against cadmium (Cd)-induced renal damage were evaluated in rats.

MATERIALS AND METHODS: Wistar大鼠是对待的d by gavage to RUT (30 mg/kg) or Se (0.15 ppm) or Cd (200 ppm) in drinking water alone or in combination (30 mg/kg RUT +0.15 ppm Se + 200 ppm Cd).玉米油被用作媒介物（2 mL/kg）。 After a 5-week treatment period, rat kidneys were removed for biochemical assays and histopathological examination.Se and Cd levels were evaluated by flame atomic absorption spectrophotometry.

RESULTS: The malondialdehyde and glutathione levels as well as superoxide dismutase and catalase activities in the Cd-treated animals were increased compared with control values (0.056 ± 0.0003 versus 0.011 ± 0.0005 μmol/mg; 0.005 ± 0.0006 versus 0.00085 ± 0.0002 μg/mg; 1.62 ± 0.09 versus 0.48 ± 0.12 units/mg; 650 ± 25 versus 361.89 ± 31 μmol H2O2/mg分别）。 Cd treatment was also associated with decreased renal Se concentration (4.19 ± 0.92 versus 7.73 ± 0.7 μg/g dry weight), increased alkaline phosphatase (0.07 ± 0.0015 versus 0.033 ± 0.0019 unit/mg), acid phosphatase (0.029 ± 0.0021 versus 0.015 ± 0.0016 unit/mg), and lactate dehydrogenase (0.032 ± 0.004 versus 0.014 ± 0.0027 unit/mg) activities, respectively, and with evidence of severe renal damage.车辙和SE或其单独效应的组合阻止了CD诱导的氧化肾脏损伤。 However, their combine effects do not have stronger effects than their separate effect against Cd-induced renal damage.

DISCUSSION AND CONCLUSION: RUT and Se function as potent antioxidant in the protection of renal damage induced by Cd.