[高剂量维生素C对急性髓样白血病细胞的增殖和凋亡的影响]。
摘要来源：
ehongguo shi yan yan xue ye xue ye xue za zhi。 2020 Jun; 28（3）：833-841。 PMID： 32552944“> 32552944 Wang, Zhu Yang, Xun Ni, Jin Wei
Article Affiliation:Xiao-Jing Lin
Abstract:OBJECTIVE: To investigate the effects of high dose vitamin C on proliferation and apoptosis of acute myeloid leukemia (AML) cell lines including HL-60, U937 and primary CD34leukemia cells in AML.
METHODS: CD34cells were sorted by using immunomagnetic cell sorting system, then the primary CD34leukemia cells, including HL-60 and U937 cell lines were cultured in vitro.每组中的细胞用差异处理维生素C的T浓度，通过MTT测定确定细胞的存活率，通过膜联蛋白V/PI双重染色评估细胞的凋亡率，包括裂解caspase 3的凋亡蛋白的表达，裂解的caspase-9，裂解caspase-9和裂解的PARP通过蛋白质blot。 class =“ sub_abstract_label”>结果： HL-60和U937细胞的增殖可以被高剂量的维生素C抑制，高剂量维生素C显示出浓度依赖性的方式（r = -0.9664; r = -0.9796）。 HL-60 and U937 cells were treated with different concentrations of vitamin C (8 and 20 mmol/L) for 24 hours, respectively, it was found that with the increasing of vitamin C concentration, cell apoptosis rate was significantly increased (r=0.9905; r=0.9971), and the expression of apoptosis related proteins including cleaved caspase 3, cleaved caspase-9 and cleaved PARP随着浓度的增加而显着增加。此外，发现有或WTET2，高剂量维生素C的突变可以抑制增殖（r = -0.9719; r = -0.9699），并促进凋亡（r = 0.9998; r = 0.9901）的原发性CD34Leukemia细胞的AML中未= dose = -0的效应，但它显示出均依赖于DOSE的CD34Leukemia细胞，但它显示出依赖于DOSE的效应。正常CD34细胞的凋亡（r = 0.1912）。
结论： 高剂量维生素C可以抑制增殖并抑制急性骨髓细胞和选择性地杀死急性骨髓细胞的细胞和选择性杀死的细胞的凋亡AML。