BMC Vet Res。 2023年10月10日； 19（1）：199。 EPUB 2023 10月10日。PMID： 37817228> 37817228 Bai，Linzheng Wang，Fuwei Zhao，Houmei Li，Dongliang Wang，Quanhai pang
文章隶属关系：shihao ge
摘要：
方法： Andro对Andro对Leghorn Male Hepatoma（LMH）（LMH）的毒性的毒性（lmh）在不同的浓度和时代分析。使用自动生物化学分析仪测量培养上清液中丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）活性，以评估Androscopolide对LPS诱导的LMH细胞损伤的保护作用。 Subsequently, TMT proteomics analysis were performed on the negative control group (NC group), LPS, and LPS-Andro groups, and bioinformatics analysis was performed on the differentially expressed proteins (DEPs).
RESULTS: It was found that Andro reduced ALT and AST levels in the cell supernatant and减轻了LPS诱导的LMH细胞损伤。蛋白质组学分析S分别在LPS与NC组和LPS Andro与LPS组中确定了50和166个差异表达的蛋白质。 Andro may be involved in steroid metabolic processes, negative regulation of MAPK cascade, oxidative stress, and other processes to protect against LPS-induced liver injury.
CONCLUSIONS: Andro protects against LPS-induced liver injury, HMGCS1, HMGCR, FDPS, PBK,通过差异蛋白质组学鉴定的CAV1，PRDX1，PRDX4和PRDX6可能是Andro的靶标。我们的研究可能为抗肝损伤的Andro保护提供新的理论支持。