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Abstract Title:Kolaviron, Biflavonoid Complex from the Seed of Garcinia kola Attenuated Angiotensin II和LypopolysacaChide诱导的血管平滑肌细胞增殖和一氧化氮的产生。
摘要来源：
Pharmagognocognosy Res。 2016年3月; 8（增刊1）：S50-5。 PMID： 27114693
atbract abracter（s） Adeolu Alex Adedapo，Momoh audu Yakubu 文章隶属关系：ademola adetokunbo oyagbemi
摘要：
recorts withy： kolaviron（kolaviron（kv）抗炎，抗氧化剂，肝保护剂，心脏保护，肾脏保护和其他阵列of chemopreventive capabilities but the mechanism of action is still not completely understood.
MATERIALS AND METHODS: In this study, we investigated the anti-proliferative, anti-inflammatory and anti-oxidative potential of KV in cultured Vascular Smooth Muscle Cells (VSMCs). KV（25-100μg/mL）单独或在使用有丝分裂原和促炎剂血管紧张素II（AG II，10（-6）M）和脂多糖（LPS，100μg/ml）的促VSMC增殖的影响以及无生产的影响。通过MTT分析确定细胞增殖，一氧化氮水平通过Griess分析确定。 KV剂量和时间依赖于减弱的VSMC生长。
结果： 用AG II和LPS的VSMC处理可显着增强该细胞的增殖，从而通过使用KV处理显着减弱。用LPS处理VSMC显着incre培养基中的ASED一氧化氮（NO）水平被KV衰减。 These results demonstrated anti-proliferative anti-inflammatory properties of KV as it clearly inhibited cellular proliferation induced by mitogens as well as LPS-induced inflammatory processes.
CONCLUSION: Therefore, KV may mitigate cardiovascular conditions that involve cell proliferation, free radical generation and高血压，糖尿病和中风等炎症过程。 However, the molecular mechanism of action of KV needs to be investigated.
SUMMARY: Angiotensin-induced cell proliferationKolaviron mitigates angiotensin-induced cell proliferationKolaviron ameliorates nitric oxide productionKolaviron offers antioxidant activity.使用的缩写：VSMC：血管平滑肌细胞，AG II：血管紧张素II，KV：Kolaviron，LPS，LPS：Lyppolysacachide，NO：一氧化氮，DMEM：Dulbeccos：Dulbeccos Modifie Modified Eagles medium, MTT: (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide), DMSO: Dimethylsulfoxide, GB1: Garcinia kola biflavonoid-1, GB2: Garcinia kola biflavonoid-2, ROS: Reactive oxygen species, ET-1: Endothelin-1, NF-κB：核因子-kappa beta，Cox-2：环氧合酶2。