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Abstract Title:Docosahexaenoic Acid (DHA) Reduces LPS-Induced Inflammatory Response Via ATF3 Transcription Factor and Stimulates Src/Syk小胶质细胞中的信号依赖性吞噬作用。
摘要来源：
细胞生理生物化学。 2023年11月13日； 57（6）：411-425。 PMID： 379622278“> 37962278 Anna Walczewska, Emilia Zgorzynska
Article Affiliation:Katarzyna Wieczorek-Szukala
Abstract:BACKGROUND/AIMS: Microglial cells play a crucial role in the development of neuroinflammation in response to harmful刺激，例如感染，缺血或损伤。然而，它们的慢性激活与神经变革烯的进展有关疾病。 Therefore, looking for potential factors limiting microglial activation, the effect of docosahexaenoic acid (DHA) on the inflammatory response and TREM2-dependent phagocytic activity in microglia was investigated.
METHODS: In LPS-induced primary microglia preincubated with DHA, or without预孵育分别通过RT-PCR和WB确定ATF3和TREM2基因和TREM2，SYK，AKT蛋白的表达。通过MTT和细胞因子测定细胞活力，趋化因子表达由蛋白质组探测器测定法确定。此外，使用免疫荧光进行了小胶质细胞的吞噬活性。
结果： 我们发现，DHA明显增加了attf3的表达，并降低了cinc-1-1的水平，cinc-2αβ，cinc-2αpin和cinc-2αciels-cincines and-33 and-cincine and-33 and-cincine and-33 and。 ICAM-1粘附蛋白。另外，小胶质细胞与DHA resulted in increased Src/Syk kinases activation associated with increased phagocytic microglia activity.
CONCLUSION: These findings indicate that DHA efficiently inhibits ATF3-dependent release of proinflammatory mediators and enhances phagocytic activity of microglia.该研究提供了反应性小胶质细胞中DHA作用的新机制，这可能有助于限制大脑促炎环境引起的神经元损害。