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Abstract Title:Anti-neuroinflammatory of Chloroform Extract ofRoot Culture on Lipopolysaccharide-stimulated BV2小胶质细胞。
摘要来源:
REP Biochem Mol Biol。 2022年4月; 11(1):125-137。 pmid: 35765526“> 35765526 Yian Koh, Sobri Hussein
Article Affiliation:Chee Hoe Cheah
Abstract:Background: It is believed that activation of microglia in the central nervous system upon detection of stimulus like lipopolysaccharides provokes neuroinflammation via the production of pro-inflammatory介质和细胞因子。各种民间医学的细胞保护和抗炎特性一直是GA注意作为对抗各种疾病的策略。 This study aimed to assess the anti-neuroinflammatory properties of chloroform extract ofroot culture based on nitric oxide and cytokines production.
Methods: The study was initiated with the determination of maximum non-toxic dose (MNTD) ofroot culture chloroform extract using the MTT assay.通过通过Griess Assay测量一氧化氧化物(NO)的产生,用TNF-α,IL-6,IL-6和IL-10和IL-10使用Qashine Elisa 结果: 发现提取物的mntd和½mntd在产生促炎性细胞因子(例如NO,TNF-α和IL-6)中没有重要作用。但是,氯仿提取物的mntd和½mntd显着增加了蚂蚁与未处理的细胞相比,I炎症的IL-10。
结论: 与此,氯仿提取物ofroot培养物可能会施加抗 - 神经毒素的特性。