lps诱导的RAW 264 7巨噬细胞中p38 MAPK和NF-κB途径的衰减，Ganoderma Lucidum固醇的抗炎作用

Juan Xu

Ganoderma lucidum exhibits pronounced anti-inflammatory effects, polysaccharides and triterpenoids are regarded as major constituents displaying the抗炎活性，固醇是否有助于这种活性尚不清楚。本文中的Ganoderma Lucidum固醇（GLS）通过单步操作进行了创新的分离，GLS的曲线以HPLC-ELSD为特征，显示与传统分离的固醇相似NAL方法，但内容更高。此外，GLS通过显着衰减LPS诱导的细胞极化以及促炎性介质的释放和mRNA表达来抑制巨噬细胞的炎症，TNF-α，IL-1β和IL-6。 Moreover, the anti-inflammatory activity of GLS was mediated by MAPK and NF-κB pathways, GLS suppressed MAPK pathways by blocking phosphorylation of p38 but not ERK and JNK, which is complementary with inhibitory effects of Ganoderma polysaccharides and triterpenes on JNK and ERK, indicating Ganoderma sterols may exert synergistic anti-inflammatory effect with多糖和三萜。 GL还通过限制IκB-α的磷酸化和降解并阻止NF-κBp65的磷酸化来抑制NF-κB途径。分子对接证实，GL的固醇直接与p38和p65的活性位点结合以抑制它们的激活。因此，我们的发现表明GL是天然和安全的抗炎剂，以防止并治疗炎症性疾病。