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摘要来源:
pharm Biol。 2013年8月; 51(8):1066-76。 EPUB 2013年6月7日。pmid: 23746221“> 23746221 Bang,年轻的儿子,Beom-tae Kim,Dong-Hern Kim,Seung-Ah Lee,Minseon Chae,Da Hye Kim,Jeong-Chae Lee
文章隶属关系:ki-chi choi
摘要:
MATERIALS AND METHODS: The macrophages were exposed to 100 μg/mL of each of the barley extracts in the presence of 1 μg/mL lipopolysaccharide (LPS)在孵育24或48小时后,通过各种测定法分析了细胞或培养上清液。还使用注射LPS的spicticmouse模型研究了大麦馏分的抗炎潜力。 The active constituents in the fractions were identified using gas chromatography-mass spectrometry (GC-MS).
RESULTS: The active fractions, named F₄, F₇, F₉ and F₁₂, inhibited almost completely the LPS-induced production of nitric oxide (NO) and inducible NO synthase.以100μg/mL的方式进行预处理,将肿瘤坏死因子-α(TNF-α)水平降低至19.8、3.5、1.2和分别为1.7 ng/mL比较了单独的TOLPS治疗(41.5 ng/ml)。这些以100μg/mL为单位的馏分显然抑制了白介素(IL)-6和IL-1β的分泌以及LPS刺激的细胞中核因子-κB的DNA结合活性。注射后48小时,注射LPS(30 mg/kg BW)的小鼠在48小时内显示20%的存活率,而口服馏分的生存率提高了生存率至80%。 GC-MS analysis revealed the presence of the derivatives of benzoic and cinnamic acids and fatty acids in the fractions.
DISCUSSION AND CONCLUSION: The aerial parts of barley are useful as functional food to prevent acute inflammatory responses.