PUER茶水提取物可预防脂多糖诱导的神经炎症的小鼠模型中的认知障碍

PUER茶水提取物可以在脂多糖诱导的神经炎症的小鼠模型中预防认知障碍。

phytomedicine。 2020年12月; 79：153338。 EPUB 2020 9月19日。PMID： 3299202081“> 32992081 Pak, Wei Li, Younghoon Go, Jung-Jin Lee

Yun Hee Jeong

BACKGROUND: Puer tea, a type of post-fermented tea made from Camellia sinensis leaves, has long been widely used in East Asian countries.它主要是在中国南部生产的，由于其分解脂肪的能力而有效预防肥胖症。然而，尚未研究pu茶对内毒素的认知障碍或神经炎症的影响。

perim> perim： 在这里，我们在这里，我们是一个ssessed the inhibitory activity of Puer tea hot water extract (PTW) on neuroinflammation and cognitive impairment and explored its mechanism.

STUDY DESIGN: The ability of PTW to inhibit cognitive impairment was investigated in a mouse model of lipopolysaccharide (LPS)-induced neuroinflammation and murine microglia BV2 cells.

METHODS: We examined whether oral administration of PTW prevented cognitive impairment and LPS-induced neuroinflammation using behavioral tests, Nissl staining, immunohistochemistry, western blotting, real-time reverse转录聚合酶链反应（实时RT-PCR），GRIESS测定和酶联免疫吸附测定（ELISA）。

“ sub_abstract_label”>结果：结果： first，莫尔斯水上（Maze and Maze and sassive）和随意的（maze the pa the p.减弱LPS诱导的水疗中心小鼠大脑的TIAL记忆丧失和抑制神经元损伤。组织病理学分析表明，PTW抑制了LPS诱导的激活标记物电离钙结合适配器分子-1（IBA-1）和神经胶质纤维纤维酸性蛋白（GFAP）的表达。此外，PTW抑制了淀粉样生成蛋白的表达，例如淀粉样蛋白-β前体蛋白（APP），C99和β-分泌酶-1（BACE-1）；产生炎性蛋白，例如IBA-1，GFAP，诱导型一氧化氮合酶（INOS）和环氧酶（COX）-2；激活炎症途径；以及海马组织中炎症介质mRNA的表达。 In cultured microglia, PTW treatment inhibited the generation of various inflammatory factors activated by LPS.

CONCLUSION: Our results in vivo and in vitro demonstrate that PTW effectively prevents cognitive impairment caused by neuroinflammation and is, therefore, a potential candidate for the develo神经退行性疾病的治疗剂。