[研究姜黄素对HSC的PDGF-BB,PDGFRBETA和ERK1表达的影响]。
摘要来源:
phy yao cai。 2009年5月; 32(5):732-5。 PMID: 19771848” 广州510520,广州毒品和毒品职业学院。 To observe the effects of curcumin on expression of PDGF-BB, PDGFRbeta and ERK1 of rat Hepatic stellate cell (HSC-T6). METHODS: The cultured HSC were divided into control group, each curcumin treating group.通过MTT分析检测到姜黄素对HSC增殖的影响。蛋白质l通过免疫组织化学检查检测到PDGF-BB,PDGFRBETA和ERK1的evels; The influence of curcumin on expression of PDGF-BB, PDGFRbeta and ERK1 mRNA was detected by RT-PCR. RESULTS: Immunohistiochemical results showed that normal cultured HSC-T6 cell expressed PDGF-BB, PDGFRbeta and ERK1 remarkably.在不同浓度的姜黄素干预后,PDGF-BB,PDGFRBETA和ERK1的男性程度明显下降。 RT-PCR结果表明,在对照和TGF-BETA1刺激组中,PDGF-BB,PDGFRBETA和ERK1 mRNA的表达显然可以看到。在不同浓度的姜黄素干预后,PDGF-BB,PDGFRBETA和ERK1 mRNA的表达以剂量依赖性的方式降低。 结论: curcumin可能会抑制pdgf的表达,pdgfrb of pdgfbb of pdgfbb,pdgfbb of pdgfbb,姜黄素对抗纤维化的机理。