烟酰胺单核苷酸减轻内毒素诱导的急性肺损伤

Simeng He

CONTEXT: Sepsis-induced acute lung injury (ALI) is a severe condition with limited effective治疗学；据报道，烟酰胺单核苷酸（NMN）发挥抗炎活性。

objeCTIVE: This study explores the potential mechanisms by which NMN ameliorates sepsis-induced ALIand.

MATERIALS AND METHODS: Cultured MH-S cells and a murine model were used to evaluate the effect of NMN on sepsis-induced ALI.用LPS（1μg/mL）和NMN（500μM）刺激MH-S细胞，以将12小时组成作为对照，LPS和LPS+NMN。检测到细胞活力，凋亡状态和M1/2巨噬细胞相关标记。 The mice were pretreated intraperitoneally with NMN (500 mg/kg) and/or EX-527 (5 mg/kg) 1 h before LPS injection and randomized into 7 groups ( = 8): control, LPS, LPS + NMN, NMN, LPS + NMN + EX-527 (a SIRT1 inhibitor), LPS + EX-527, and EX-527. 12小时后，检测到肺组织病理学，W/D比，MPO活性，NADAND ATP水平，M1/2巨噬细胞相关标记以及SIRT1/NF-κB途径的表达。

sub_abstract_label“>伪造的凋亡率从12.25％降低到5.74％。在化粪池小鼠中，NMN改善了肺中的典型病理发现，W/D比和MPO活性降低，但NADAND ATP水平升高。此外，NMN抑制了M1，但促进了M2极化，并上调了SIRT1的表达，并抑制了NF-κB-P65乙酰化和磷酸化。 Furthermore, inhibition of SIRT1 reversed the effects of NMN-induced M2 macrophage polarization.

CONCLUSIONS: NMN protects against sepsis-induced ALI by promoting M2 macrophage polarizationthe SIRT1/NF-κB pathway, it might be an effective strategy for preventing或处理败血症诱导的ali。