加密省会通过调节巨噬细胞的代谢重编程来减轻LPS诱导的急性肺损伤

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背景：背景： 急性肺损伤（Ali），而没有生命的疾病，有效地有效炎性疾病。巨噬细胞极化在肺部炎症的启动和分辨率中起关键作用。因此，调节巨噬细胞表型是急性LUN的潜在有效方法G受伤。加密酮（CTS）是一种亲脂性生物活性化合物，它是从多种药理作用的根部提取的，尤其是抗炎作用。 In this study, we investigated the therapeutic and immunomodulatory effects of CTS on ALI.

MATERIALS AND METHODS: The rat model of ALI was established by intratracheal instillation of LPS (5 mg/kg) to evaluate the lung protective effect of CTSand to explore the regulation of CTS在肺巨噬细胞极化的表型上。 LPS (1μg/mL) was used to stimulate RAW264.7 macrophagesto further explore the effect of CTS on the polarization and metabolic reprogramming of RAW264.7 macrophages and to clarify the potential mechanism of CTS anti-ALI.

RESULTS: CTS significantly improved lung功能，减少肺水肿，有效抑制肺部炎症性浸润，并减轻ALI。 bOthandresults表明，CTS抑制了巨噬细胞分化为M1表型，并在ALI期间促进了极化为M2表型。进一步表明，CTS显着抑制了LPS诱导的从有氧氧化到巨噬细胞糖酵解的代谢过渡。从机械上讲，CTS通过激活AMPK来阻止LPS诱导的巨噬细胞的代谢转化。

结论： 这些发现表明CTS通过激活AMPK和MACR oppy tospe tospepe tosemape to to tospepe tosive to ym2-M1-MACR-MACR-M1---M1----M1----M1----M1---M1---M1----M1---M1---巨噬细胞，从而减轻了ALI的炎症反应，这表明CTS可能是潜在的抗ALI药物。