白藜芦醇在体外减轻氧化应激和诱变

氧化性DNA修饰，例如7,8-二氢-8-氧气（8-oxog），在显然所有活细胞中都会产生。在CSB（M/M）OGG1⁻（/）⁻小鼠中修复8-oxog的缺陷会导致这些病变的基础水平升高，并增加自发突变的频率，如果刺激细胞增殖，则会在肝脏中造成肿瘤发生。 Here, we describe that the phytoalexin resveratrol, applied either for 7 days per gavage (100 mg/kg bodywt) or for 3-9 months in the diet (0.04% ad libitum), reduces the endogenous oxidative DNA base damage in the livers of the Csb(m/m)Ogg1⁻(/)⁻ mice by 20-30% (P<0.01).在野生型动物中也观察到很小但一致的作用。在BigBlue®CSB（M/M）OGG1⁻（/）⁻小鼠的LACI基因中确定的自发突变频率被白藜芦醇伴随降低至类似的扩展。 Mechanistically, the protection is caused by an induction of the antioxidant defense system since (i) hepatocytes isolated from all resveratrol-treated animals were less susceptible to the generation of single-strand breaks and to cell killing by H₂O₂, (ii) messenger RNA levels of superoxide dismutases 1 and 2 (SOD1 and SOD2) heme oxygenase-1 and glutathione peroxidase were短期治疗和（iii）突变的显着上调，主要归因于氧化基碱修饰8-oxog（g：c至t：a transtersions）比g：c到a：t跃迁归因于自发脱氨基的t跃迁更为强烈地抑制。因此，结果表明，通过应用外源性剂可以减少内源性氧化DNA损伤产生的自发体细胞突变率。