在未经蟾蜍(bufo viridis)胃的肌肉和低能激光照射促进该过程的
再生。
摘要来源:
anat rec。 1995年1月; 241(1):123-8。 PMID: 7879917“> 7879917
atbract austresp austrative(s) bibikova 摘要:
背景: ,众所周知,在这种条件下,骨骼肌肉不得能够再生,但在这种情况下不会发生再生的肌纤维的成熟。神经支配还会导致“侵入性”和卫星细胞的升高,但是这些细胞在损伤死于肌肉后的再生过程中的作用仍然未知。最近发现低能激光器可以调节和加速细胞的生理过程。本研究的目的是比较Denervate的再生d and innervated amphibian muscles and to investigate whether this process in denervated muscles can be stimulated by low energy laser irradiation prior to injury in these muscles.
METHODS: Denervated gastrocnemius muscles of toads were irradiated with He-Ne laser (6.0 mW, 31.2 J/CM2)7天后(对照肌肉在相同的波长下接受红光照射)。在两组肌肉的照射部位进行神经支配后九天进行冷损伤。 At 14 days postinjury all muscles were removed and processed for histology and histomorphometric analysis of mononucleated cells, myotubes, and young myofibres in the regenerated zone.
RESULTS: The volume fraction (percent of total injured zone) of the various histological structures in the injured zones 14在被剥离的几天(在受伤前9天)肌肉后几天没有DI从神经受伤的肌肉进行间隔间隔后的肌肉。激光辐照肌肉中的单核细胞和肌管分别包括49 +/- 4%和6 +/- 1%的受伤区域,其体积分数明显低于其体积分数(分别为67 +/- 2%和11 +/- 2%)。 The young myofibres populated 34 +/- 4% of the total injured area in the denervated and laser irradiated muscles which was significantly higher than their volume fraction (12 +/- 2%) in control denervated muscles.
CONCLUSIONS: It is concluded that initial stages of regeneration can also take place在两栖动物的骨骼中被剥夺和受伤的肌肉。再生过程的动力学在未经神经和神经支配的肌肉中相同。如果肌肉在受伤之前被低能量激光辐射,可以显着增强未经肌肉的再生过程,可能是通过Activa“招募”并参与再生过程的肌肉中的细胞(刺激增殖和/或分化)细胞。