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Abstract Title:Salvianolic acid B ameliorates myocardial fibrosis in diabetic cardiomyopathy by去泛素化Smad7。
抽象来源：
下巴医学。 2023年12月10日； 18（1）：161。 EPUB 2023 12月10日。PMID： 38072948> 38072948 Wang, Yini Xu, Ling Tao, Xiangchun Shen
Article Affiliation:Hong Luo
Abstract:BACKGROUND: Salvianolic acid B (Sal B), a water-soluble phenolic compound derived from Salvia miltiorrhiza Bunge, is commonly used in Traditional治疗心血管疾病的中药。在我们先前的研究中，SAL B受到糖尿病心肌病（DCM）诱导的心肌纤维化的保护。这项研究旨在调查the ameliorative effects and potential mechanisms of Sal B in mitigating myocardial fibrosis induced by DCM.
METHODS: Various methods were used to investigate the effects of Sal B on myocardial fibrosis induced by DCM in vivo and in vitro. These methods included blood glucose measurement, echocardiography, HE staining, Massons trichrome staining, Sirius red staining, cell proliferation assessment, determination of hydroxyproline levels, immunohistochemical staining, evaluation of fibrosis-related protein expression (Collagen-I, Collagen-III, TGF-β1, p-Smad3, Smad3, Smad7, andα-smooth muscle actin), analysis of Smad7 gene expression, and analysis of Smad7 ubiquitin modification.
RESULTS: The animal test results indicated that Sal B significantly improved cardiac function, inhibited collagen deposition and phenotypic transformation, and ameliorated myocardial fibrosis inDCM通过上调SMAD7，从而抑制TGF-β1信号通路。此外，细胞实验表明，SAL B显着抑制了高葡萄糖（HG）诱导的心脏成纤维细胞（CFS）的增殖，迁移，表型转化和胶原蛋白分泌。 SAL B显着降低了Smad7的泛素化并稳定了Smad7的蛋白质表达，从而增加了CFS中Smad7的蛋白质表达并抑制TGF-β1信号通路，这可能是SAL通过该机制来缓解dcm诱导的心肌纤维化的潜在机制。 class =“ sub_abstract_label”>结论： 这项研究表明，SAL B可以通过去泛素化SMAD7，稳定SMAD7的蛋白质表达并阻止TGF-β1信号通路来改善DCM中的心肌纤维化。