theaflavin促进鼻咽癌的凋亡

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Theaflavin promoted apoptosis in nasopharyngeal carcinoma unexpectedly via inducing自噬。

伊朗J基本科学。 2022年1月； 25（1）：68-74。 PMID： 35656444“> 35656444 Guo，Hong GE

jing xu

目标： 本研究旨在研究鼻虫（TF）在鼻虫（TF）抗癌效应的机制class =“ sub_abstract_label”>材料和方法： CNE2细胞用于研究TF的抗癌作用。这项研究使用细胞计数套件8（CCK8）测定在增殖和流式细胞仪上进行DETECT凋亡。 The protein expression of Bcl-2, Bax, caspase 3, and caspase 9 was detected by Western blot, and autophagy-related proteins were also detected.

Results: TF inhibited proliferation of CNE2 cells, promoted apoptosis, and up-regulated the expression of caspase 3, caspase 9和Bax，并降低了Bcl-2的水平。出乎意料的是，TF通过上调自噬标记光链3（LC3）和与溶酶体相关的膜蛋白1（LAMP1）的水平以及降低自噬量货物量蛋白p62的水平，并通过MTOR路径来降低，TF引起了自噬而不是抑制自噬。 Besides, autophagy inhibitor Chloroquine (CQ) suppressed the effect of TF on Bax, Bcl-2 and activation of caspase 3 and caspase 9.

Conclusion: TF promoted apoptosis of nasopharyngeal carcinoma cells, the mechanism was unexpectedly involved in inducing autophagy。